Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.
Uniprot primary ID | P04049 |
PDB ID | 3O8I 3KUD 1RFA 4IEA 1FAR 3IQJ 3KUC 3CU8 3IQV 3IQU 4IHL 1GUA 3NKX 4G0N 4FJ3 4G3X 1FAQ 1C1Y 3OMV |
DrugBank ID | DB00398 DB08896 DB08912 |
BioGrid ID | 111831 |
GuidetoPHARMACOLOGY ID | 2184 |
PharmGKB ID | PA34183 |
KEGG ID | hsa:5894 |
DIP ID | DIP-1048N |
STRING ID | 9606.ENSP00000251849 |
IntAct ID | P04049 |
DMDM | 125651 |
BRENDA | 2.7.10.2 |
Reactome | R-HSA-5675221 R-HSA-5674499 R-HSA-5674135 R-HSA-442742 R-HSA-5621575 R-HSA-430116 R-HSA-2672351 R-HSA-392517 R-HSA-5673000 |
SignaLink | P04049 |
BioCyc | |
Entrez Gene (Gene ID) | 5894 |
BindingDB | P04049 |
Fingerprint type | Sensitivity | SEN_std | Specificity | SPE_std | Accuracy | ACC_std | F1-score | F1-score_std | AUC | AUC_std | MCC | MCC_std | Download model |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
FP2 fingerprints | 0.845 | 0.005 | 0.931 | 0.005 | 0.888 | 0.004 | 0.885 | 0.005 | 0.951 | 0.003 | 0.783 | 0.008 | Download |
Estate fingerprints | 0.821 | 0.006 | 0.855 | 0.004 | 0.838 | 0.004 | 0.835 | 0.005 | 0.910 | 0.000 | 0.674 | 0.007 | Download |
MACCS fingerprints | 0.820 | 0.000 | 0.856 | 0.006 | 0.838 | 0.004 | 0.834 | 0.005 | 0.921 | 0.003 | 0.674 | 0.005 | Download |
Daylight fingerprints | 0.732 | 0.009 | 0.950 | 0.007 | 0.841 | 0.006 | 0.825 | 0.008 | 0.918 | 0.004 | 0.702 | 0.011 | Download |
ECFP2 fingerprints | 0.881 | 0.003 | 0.907 | 0.005 | 0.894 | 0.005 | 0.894 | 0.005 | 0.960 | 0.000 | 0.791 | 0.007 | Download |
ECFP4 fingerprints | 0.867 | 0.005 | 0.959 | 0.005 | 0.913 | 0.005 | 0.908 | 0.004 | 0.970 | 0.000 | 0.829 | 0.009 | Download |
ECFP6 fingerprints | 0.832 | 0.008 | 0.974 | 0.003 | 0.903 | 0.005 | 0.896 | 0.005 | 0.964 | 0.005 | 0.817 | 0.009 | Download |
Positive dataset | Negative dataset |
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E-mail: biomed@csu.edu.cn